Hotspot

ycf16

SSC · 119536-120312 · 777 bp

Back to Saccharina

Pi 0.0242

MarkerSeek score 52.60

Primer available yes

Label ycf16

Feature Metadata

label_name: ycf16
feature_type: gene
region: SSC
length_bp: 777
variable_sites: 35
pi: 0.0242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf16_p1	CCACTTCGAAAGTTGGGGAA	AAGCTCCCCAGGTAGGATTT	50.0	50.0	58.0	58.0	1280-1293 bp	1.0	83.8
ycf16_p2	TTCCACTTCGAAAGTTGGGG	AAGCTCCCCAGGTAGGATTT	50.0	50.0	58.0	58.0	1282-1295 bp	1.0	83.8
ycf16_p3	AACCAATTACACGCGGCTAT	AAGCTCCCCAGGTAGGATTT	45.0	50.0	58.0	58.0	1806 bp	0.5	59.6
ycf16_p4	ACCAATTACACGCGGCTATT	AAGCTCCCCAGGTAGGATTT	45.0	50.0	58.0	58.0	1805 bp	0.5	59.6
ycf16_p5	TAGAAGGTTGTACAGCCCCA	AAGCTCCCCAGGTAGGATTT	50.0	50.0	58.0	58.0	1838 bp	0.5	59.6