Hotspot

psbC

SSC · 121882-123306 · 1425 bp

Back to Saccharina

Pi 0.00772

MarkerSeek score 39.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: SSC
length_bp: 1425
variable_sites: 20
pi: 0.00772
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCAAACCGTTTTTGGTCACA	TGAAAAGCTGTCGGGTCAAA	45.0	45.0	58.0	58.0	2432 bp	1.0	81.5
psbC_p2	TCCAAATTGGTGGTCTGTGG	TGAAAAGCTGTCGGGTCAAA	50.0	45.0	58.0	58.0	2863 bp	1.0	81.4
psbC_p3	ATGCTGCGAATACTTTCCGT	TGAAAAGCTGTCGGGTCAAA	45.0	45.0	58.0	58.0	2497 bp	1.0	81.2
psbC_p4	TCCGTGCATTTACACCAACA	TGAAAAGCTGTCGGGTCAAA	45.0	45.0	58.0	58.0	2482 bp	1.0	81.2
psbC_p5	GTTTTGGGGTAGCTGCGATA	TGAAAAGCTGTCGGGTCAAA	50.0	45.0	58.0	58.0	2662 bp	1.0	81.1