Hotspot

trnQ-ycf65

SSC · 128486-129017 · 532 bp

Back to Saccharina

Pi 0.0486

MarkerSeek score 67.20

Primer available yes

Label trnQ-ycf65

Feature Metadata

label_name: trnQ-ycf65
feature_type: igs
region: SSC
length_bp: 532
variable_sites: 47
pi: 0.0486
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-ycf65_p1	CTTTGACTCCGCCATTCGTA	TCAAGTTTGAACTTTTGGCT	50.0	35.0	58.0	53.8	597-603 bp	1.0	53.6
trnQ-ycf65_p2	TTTGACTCCGCCATTCGTAG	TCAAGTTTGAACTTTTGGCT	50.0	35.0	58.0	53.8	596-602 bp	1.0	53.6
trnQ-ycf65_p3	CTTTGACTCCGCCATTCGTA	TTCAAGTTTGAACTTTTGGCT	50.0	33.3	58.0	54.7	598-604 bp	1.0	52.6
trnQ-ycf65_p4	TTTGACTCCGCCATTCGTAG	TTCAAGTTTGAACTTTTGGCT	50.0	33.3	58.0	54.7	597-603 bp	1.0	52.6
trnQ-ycf65_p5	AGGTTCGAATCCTCCTACCC	TCAAGTTTGAACTTTTGGCT	55.0	35.0	58.2	53.8	578-584 bp	1.0	52.2