Hotspot

trnT-trnV

SSC · 116877-117159 · 283 bp

Back to Saccharina

Pi 0.102

MarkerSeek score 74.00

Primer available yes

Label trnT-trnV

Feature Metadata

label_name: trnT-trnV
feature_type: igs
region: SSC
length_bp: 283
variable_sites: 51
pi: 0.102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnV_p1	TTCAAGTCCTTTCACCAGCT	GTGACCCTCTGCTTGTAAGG	45.0	55.0	57.0	57.9	322-376 bp	1.0	83.0
trnT-trnV_p2	TTCAAGTCCTTTCACCAGCT	CTGGACTTGAACCAGTGACC	45.0	55.0	57.0	58.1	336-390 bp	1.0	82.8
trnT-trnV_p3	GTTCAAGTCCTTTCACCAGC	GTGACCCTCTGCTTGTAAGG	50.0	55.0	56.9	57.9	323-377 bp	1.0	82.7
trnT-trnV_p4	GTTCAAGTCCTTTCACCAGC	CTGGACTTGAACCAGTGACC	50.0	55.0	56.9	58.1	337-391 bp	1.0	82.5
trnT-trnV_p5	TTCAAGTCCTTTCACCAGCT	GACTTGAACCAGTGACCCTC	45.0	55.0	57.0	57.8	333-387 bp	1.0	82.4