Hotspot

rpl11-trnW

SSC · 109959-110068 · 110 bp

Back to Saccharina

Pi 0.0409

MarkerSeek score 63.70

Primer available yes

Label rpl11-trnW

Feature Metadata

label_name: rpl11-trnW
feature_type: igs
region: SSC
length_bp: 110
variable_sites: 8
pi: 0.0409
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl11-trnW_p1	AGCTTTCCCTGCAGATAACG	AACCTAGTGTCGAGGGTTCA	50.0	50.0	58.0	58.0	200-201 bp	1.0	82.9
rpl11-trnW_p2	AGCTTTCCCTGCAGATAACG	ACCTAGTGTCGAGGGTTCAA	50.0	50.0	58.0	58.0	199-200 bp	1.0	82.9
rpl11-trnW_p3	AGCTTTCCCTGCAGATAACG	GTCGAGGGTTCAACTCCTTC	50.0	55.0	58.0	57.9	192-193 bp	1.0	82.4
rpl11-trnW_p4	AGCTTTCCCTGCAGATAACG	CGAGGGTTCAACTCCTTCAG	50.0	55.0	58.0	57.9	190-191 bp	1.0	82.4
rpl11-trnW_p5	AGCTTTCCCTGCAGATAACG	CCAAAACCTAGTGTCGAGGG	50.0	55.0	58.0	57.9	204-205 bp	1.0	82.1