Hotspot

psaL-rbcR

LSC · 79714-79764 · 51 bp

Back to Saccharina

Pi 0.0621

MarkerSeek score 65.60

Primer available yes

Label psaL-rbcR

Feature Metadata

label_name: psaL-rbcR
feature_type: igs
region: LSC
length_bp: 51
variable_sites: 5
pi: 0.0621
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaL-rbcR_p1	TGGAGGCATTAGTTTTGCGT	GCTGGGTTAGGGGTTTCATT	45.0	50.0	58.1	57.8	325 bp	1.0	80.8
psaL-rbcR_p2	CTTCGGTTGGGCACTTATCA	GCTGGGTTAGGGGTTTCATT	50.0	50.0	57.9	57.8	680 bp	1.0	80.3
psaL-rbcR_p3	CACCAGTCACTTCATCAGCA	GCTGGGTTAGGGGTTTCATT	50.0	50.0	57.8	57.8	659 bp	1.0	79.9
psaL-rbcR_p4	ACACCAGTCACTTCATCAGC	GCTGGGTTAGGGGTTTCATT	50.0	50.0	57.8	57.8	660 bp	1.0	79.9
psaL-rbcR_p5	TCGGTTGGGCACTTATCAAC	GCTGGGTTAGGGGTTTCATT	50.0	50.0	58.2	57.8	678 bp	1.0	79.7