Hotspot

clpC-trnK

LSC · 72481-72618 · 138 bp

Back to Saccharina

Pi 0.0918

MarkerSeek score 63.60

Primer available yes

Label clpC-trnK

Feature Metadata

label_name: clpC-trnK
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 23
pi: 0.0918
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC-trnK_p1	GACCTTTACGTCGTGCTCTT	AATGTGTTCGTGCTTGTCCT	50.0	45.0	57.9	58.0	781-782 bp	1.0	83.5
clpC-trnK_p2	GACCTTTACGTCGTGCTCTT	CAATGTGTTCGTGCTTGTCC	50.0	50.0	57.9	58.0	782-783 bp	1.0	83.5
clpC-trnK_p3	GACCTTTACGTCGTGCTCTT	TGTGTTCGTGCTTGTCCTAC	50.0	50.0	57.9	58.1	779-780 bp	1.0	82.7
clpC-trnK_p4	GGTGGTGAAACAGGGGATAC	AATGTGTTCGTGCTTGTCCT	55.0	45.0	57.9	58.0	1061-1062 bp	1.0	82.6
clpC-trnK_p5	GGTGGTGAAACAGGGGATAC	CAATGTGTTCGTGCTTGTCC	55.0	50.0	57.9	58.0	1062-1063 bp	1.0	82.6