Hotspot

rpl20-ilvH

LSC · 47384-47469 · 86 bp

Back to Saccharina

Pi 0.0659

MarkerSeek score 66.70

Primer available yes

Label rpl20-ilvH

Feature Metadata

label_name: rpl20-ilvH
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 10
pi: 0.0659
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-ilvH_p1	AGACCCTTCAGCTTTTTCTGT	CCTCCTGCATCCTTTTCAACT	42.9	47.6	57.4	58.2	173 bp	1.0	62.3
rpl20-ilvH_p2	AGACCCTTCAGCTTTTTCTGT	TCCTCCTGCATCCTTTTCAAC	42.9	47.6	57.4	58.2	174 bp	1.0	62.3
rpl20-ilvH_p3	AGACCCTTCAGCTTTTTCTGT	TCCTCCTGCATCCTTTTCAA	42.9	45.0	57.4	56.7	174 bp	1.0	61.7
rpl20-ilvH_p4	AGACCCTTCAGCTTTTTCTGT	CCTCCTGCATCCTTTTCAAC	42.9	50.0	57.4	56.7	173 bp	1.0	61.4
rpl20-ilvH_p5	AGACCCTTCAGCTTTTTCTGT	ATCCTCCTGCATCCTTTTCA	42.9	45.0	57.4	56.5	175 bp	1.0	59.8