Hotspot

trnY-LJCPDNA_020

LSC · 27330-27391 · 62 bp

Back to Saccharina

Pi 0.0565

MarkerSeek score 71.00

Primer available yes

Label trnY-LJCPDNA_020

Feature Metadata

label_name: trnY-LJCPDNA_020
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 6
pi: 0.0565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-LJCPDNA_020_p1	AAGGGGGCGGATTGTAAATC	AGGTCGAGATGGTCCTGATT	50.0	50.0	57.9	57.8	198 bp	1.0	80.6
trnY-LJCPDNA_020_p2	AATCCGCTGGTTTTCCTACG	AGGTCGAGATGGTCCTGATT	50.0	50.0	58.3	57.8	182 bp	1.0	79.2
trnY-LJCPDNA_020_p3	GCTGGTTTTCCTACGTTGGT	AGGTCGAGATGGTCCTGATT	50.0	50.0	58.4	57.8	177 bp	1.0	78.2
trnY-LJCPDNA_020_p4	GTTCAAATCCAACTCGGCCT	AGGTCGAGATGGTCCTGATT	50.0	50.0	58.5	57.8	159 bp	1.0	78.0
trnY-LJCPDNA_020_p5	GTTAAAGGGGGCGGATTGTA	AGGTCGAGATGGTCCTGATT	50.0	50.0	57.6	57.8	202 bp	1.0	77.7