Hotspot

atpG-atpH

LSC · 11906-12016 · 111 bp

Back to Saccharina

Pi 0.0841

MarkerSeek score 75.60

Primer available yes

Label atpG-atpH

Feature Metadata

label_name: atpG-atpH
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 15
pi: 0.0841
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpG-atpH_p1	TCAAAATCGAATAGTCCTCCAGT	TTCGCCTTCATGGAAGCTTT	39.1	45.0	57.3	58.1	260 bp	1.0	54.5
atpG-atpH_p2	TCAAAATCGAATAGTCCTCCAGT	TTTCGCCTTCATGGAAGCTT	39.1	45.0	57.3	58.1	261 bp	1.0	54.5
atpG-atpH_p3	CCATCAAAATCGAATAGTCCTCC	TTCGCCTTCATGGAAGCTTT	43.5	45.0	57.1	58.1	263 bp	1.0	52.4
atpG-atpH_p4	CCATCAAAATCGAATAGTCCTCC	TTTCGCCTTCATGGAAGCTT	43.5	45.0	57.1	58.1	264 bp	1.0	52.4
atpG-atpH_p5	TCAAAATCGAATAGTCCTCCAGTT	TTCGCCTTCATGGAAGCTTT	37.5	45.0	58.0	58.1	260 bp	1.0	51.5