Hotspot

ycf1

SSC · 124172-129760 · 5589 bp

Back to Sabulina

Pi 0.0489

MarkerSeek score 68.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5589
variable_sites: 270
pi: 0.0489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAATTCTCTACGACGCCTA	AAAGAGATCCCGGATCCGAT	50.0	50.0	57.5	58.0	615 bp	1.0	81.4
ycf1_p2	GCAATTCTCTACGACGCCTA	TCCCGGATCCGATAGTCAAA	50.0	50.0	57.5	57.9	608 bp	1.0	81.0
ycf1_p3	GCAATTCTCTACGACGCCTA	ATTGACCCCACTCGTTTGTC	50.0	50.0	57.5	58.1	247 bp	0.5	55.2
ycf1_p4	GCAATTCTCTACGACGCCTA	AGATCCCGGATCCGATAGTC	50.0	55.0	57.5	57.9	611 bp	0.5	55.1
ycf1_p5	GCAATTCTCTACGACGCCTA	GAGATCCCGGATCCGATAGT	50.0	55.0	57.5	57.9	612 bp	0.5	55.1