Hotspot

rpl32-trnL-UAG

SSC · 113836-114620 · 785 bp

Back to Sabulina

Pi 0.105

MarkerSeek score 80.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 785
variable_sites: 81
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1601 bp	0.5	59.5
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1600 bp	0.5	59.5
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	930 bp	0.5	58.8
rpl32-trnL-UAG_p4	GGGCAGCGTTAAAAGCTTTT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	57.9	58.0	1593 bp	0.5	58.5
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCTTTT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	57.9	58.0	1592 bp	0.5	58.5