Hotspot

ndhF-rpl32

SSC · 112965-113661 · 697 bp

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Pi 0.114

MarkerSeek score 72.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 697
variable_sites: 50
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAATTGGGCCGATTTTG	TTAACGCTGCCCAATATCCC	50.0	50.0	58.0	58.0	1520 bp	0.5	59.6
ndhF-rpl32_p2	TTGTTGAATCGACAGCACCA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.0	58.0	971 bp	0.5	59.5
ndhF-rpl32_p3	TGTTGCTGTCGGAAAAAGGA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.0	58.0	884 bp	0.5	59.4
ndhF-rpl32_p4	GAAATTGGGCCGATTTTGCA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.9	58.0	1518 bp	0.5	59.1
ndhF-rpl32_p5	TCGACAGCACCATCGAAAAA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	963 bp	0.5	58.8