Hotspot

psbE-petL

LSC · 65176-66451 · 1276 bp

Back to Sabulina

Pi 0.0718

MarkerSeek score 73.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 62
pi: 0.0718
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.9	57.5	1050-1437 bp	1.0	75.8
psbE-petL_p2	AGAACGTTCTCCAGTGCTTC	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.8	57.5	973-1360 bp	1.0	75.3
psbE-petL_p3	GTTCTCCAGTGCTTCCAGAC	GGAAGTTGTAGTTAAAGCCGC	55.0	47.6	58.2	57.5	968-1355 bp	1.0	75.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GGAAGTTGTAGTTAAAGCCGC	45.0	47.6	58.2	57.5	1059-1446 bp	1.0	74.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.8	57.5	1058-1445 bp	1.0	74.7