Hotspot

pafII-cemA

LSC · 60815-61523 · 709 bp

Back to Sabulina

Pi 0.0797

MarkerSeek score 80.10

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 53
pi: 0.0797
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	TTTCTATGGAATCGCAGGCC	GATCCACCAGGGCAAAAAGA	50.0	50.0	58.0	58.1	1103-1133 bp	1.0	86.9
pafII-cemA_p2	TTTCTATGGAATCGCAGGCC	AAACGATAGCACGATCCACC	50.0	50.0	58.0	58.1	1145 bp	0.5	59.2
pafII-cemA_p3	CCCGTCGTGTCCTTTATCTG	AAACGATAGCACGATCCACC	55.0	50.0	58.1	58.1	933 bp	0.5	58.9
pafII-cemA_p4	CCGTCGTGTCCTTTATCTGG	AAACGATAGCACGATCCACC	55.0	50.0	58.1	58.1	932 bp	0.5	58.9
pafII-cemA_p5	CGTTGGGGATTTCCTGGAAA	AAACGATAGCACGATCCACC	50.0	50.0	58.1	58.1	1030 bp	0.5	58.8