Hotspot

ndhC-trnV-UAC

LSC · 50663-51768 · 1106 bp

Back to Sabulina

Pi 0.071

MarkerSeek score 79.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 74
pi: 0.071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAGAAAGTTTCTCCGGCCC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.0	58.1	1239-1270 bp	1.0	87.9
ndhC-trnV-UAC_p2	CTCACTAAAGAAGGCGCCAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.0	58.1	1805-1833 bp	1.0	85.4
ndhC-trnV-UAC_p3	CTAGAAAGTTTCTCCGGCCC	GCACAAGGGCTATAGCTCAG	55.0	55.0	58.0	58.1	1890-1905 bp	1.0	85.3
ndhC-trnV-UAC_p4	TTTAGGTTCCGGCATTTGCT	CCGAGAAAGTCTACGGTTCG	45.0	55.0	58.1	58.1	1833-1861 bp	1.0	84.8
ndhC-trnV-UAC_p5	CTCACTAAAGAAGGCGCCAT	GCACAAGGGCTATAGCTCAG	50.0	55.0	58.0	58.1	2456-2468 bp	1.0	84.0