Hotspot

trnF-GAA-ndhJ

LSC · 48221-48971 · 751 bp

Back to Sabulina

Pi 0.0723

MarkerSeek score 83.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 52
pi: 0.0723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGGTAGGATG	50.0	55.0	58.1	58.0	840-863 bp	1.0	87.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	839-862 bp	1.0	87.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTAAAACGTATT	50.0	45.0	58.1	58.1	866-889 bp	1.0	86.8
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGGTAGGATGGCC	50.0	55.0	58.1	58.2	837-860 bp	1.0	85.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCCGCGCCTAAAACGTATTT	50.0	45.0	58.1	58.3	865-888 bp	1.0	85.0