Hotspot

pafI-trnS-GGA

LSC · 44133-44909 · 777 bp

Back to Sabulina

Pi 0.0889

MarkerSeek score 85.70

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 66
pi: 0.0889
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TGGCCACAATTGTAAAGGTCT	CCCTCGGTAAACAAAAGCCT	42.9	50.0	57.8	58.1	871-902 bp	1.0	78.5
pafI-trnS-GGA_p2	TGGCCACAATTGTAAAGGTCT	TTCCAATGCTACGCCTTGAA	42.9	45.0	57.8	57.8	842-873 bp	1.0	78.0
pafI-trnS-GGA_p3	TGGCCACAATTGTAAAGGTCT	ACGGAAAGAGAGGGATTCGA	42.9	50.0	57.8	58.2	892-923 bp	1.0	77.8
pafI-trnS-GGA_p4	TGGCCACAATTGTAAAGGTCT	ACCCTCGGTAAACAAAAGCC	42.9	50.0	57.8	58.4	872-903 bp	1.0	76.1
pafI-trnS-GGA_p5	TGGCCACAATTGTAAAGGTCT	AGTTCCAATGCTACGCCTTG	42.9	50.0	57.8	58.5	844-875 bp	1.0	75.1