Hotspot

rpoB-trnC-GCA

LSC · 26266-27281 · 1016 bp

Back to Sabulina

Pi 0.0814

MarkerSeek score 81.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 79
pi: 0.0814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TGGTTTGTTCTTGCCTCTCC	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1344-1374 bp	1.0	86.2
rpoB-trnC-GCA_p2	TGGTTTGTTCTTGCCTCTCC	AGCACGTATCAAGAGACCCT	50.0	50.0	58.0	58.1	1453-1484 bp	1.0	86.0
rpoB-trnC-GCA_p3	CCCCTACACAAGCAAACTGT	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1752-1782 bp	1.0	84.8
rpoB-trnC-GCA_p4	CCCCTACACAAGCAAACTGT	ATTACCCCACACCGAATTGG	50.0	50.0	58.0	57.9	2044 bp	0.5	58.7
rpoB-trnC-GCA_p5	TGGTTTGTTCTTGCCTCTCC	ATTACCCCACACCGAATTGG	50.0	50.0	58.0	57.9	1636 bp	0.5	58.7