Hotspot

trnS-GCU-trnG-UCC

LSC · 8003-8693 · 691 bp

Back to Sabulina

Pi 0.113

MarkerSeek score 83.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 77
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	815-911 bp	1.0	59.1
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	814-910 bp	1.0	58.5
trnS-GCU-trnG-UCC_p3	CACGGAAAGAGAGGGATTCG	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.1	57.3	818-914 bp	1.0	58.0
trnS-GCU-trnG-UCC_p4	CACGGAAAGAGAGGGATTCG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.1	57.3	817-913 bp	1.0	58.0
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	816-912 bp	1.0	57.4