Hotspot

ndhA

SSC · 126700-128908 · 2209 bp

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Pi 0.00775
MarkerSeek score 52.20
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2209
variable_sites
49
pi
0.00775
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 AGTCATGAATCTAAAATATTGTATTCC TGGGAGAAGTTGATCGTTGA 25.9 45.0 54.2 56.5 2244-2288 bp 1.0 42.5
ndhA_p2 GTCATGAATCTAAAATATTGTATTCCT TGGGAGAAGTTGATCGTTGA 25.9 45.0 54.2 56.5 2243-2287 bp 1.0 42.5
ndhA_p3 AGTCATGAATCTAAAATATTGTATTCC TGGGAGAAGTTGATCGTTGAA 25.9 42.9 54.2 57.2 2244-2288 bp 1.0 42.5
ndhA_p4 GTCATGAATCTAAAATATTGTATTCCT TGGGAGAAGTTGATCGTTGAA 25.9 42.9 54.2 57.2 2243-2287 bp 1.0 42.5
ndhA_p5 GTCATGAATCTAAAATATTGTATTCC TGGGAGAAGTTGATCGTTGA 26.9 45.0 53.0 56.5 2243-2287 bp 1.0 42.5