Hotspot

ccsA-ndhD

SSC · 122238-122509 · 272 bp

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Pi 0.0361
MarkerSeek score 71.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
272
variable_sites
25
pi
0.0361
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATGCAGCTCTTTTATGCGGA TTCTCGATGGCTTCTCTTGC 45.0 50.0 58.0 58.0 1143-1207 bp 0.888889 81.6
ccsA-ndhD_p2 TGCAGCTCTTTTATGCGGAT TTCTCGATGGCTTCTCTTGC 45.0 50.0 58.0 58.0 1142-1206 bp 0.888889 81.6
ccsA-ndhD_p3 ATGCAGCTCTTTTATGCGGA CTCCCATGGATTCATTGGGG 45.0 55.0 58.0 58.0 1276-1340 bp 0.888889 81.3
ccsA-ndhD_p4 TGCAGCTCTTTTATGCGGAT CTCCCATGGATTCATTGGGG 45.0 55.0 58.0 58.0 1275-1339 bp 0.888889 81.3
ccsA-ndhD_p5 TGCAGCTCTTTTATGCGGAT CTCGATGGCTTCTCTTGCAT 45.0 50.0 58.0 58.0 1140-1204 bp 0.888889 81.3