Hotspot

trnL-UAG-ccsA

SSC · 121129-121241 · 113 bp

Back to Sabia

Pi 0.0354
MarkerSeek score 65.90
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
113
variable_sites
14
pi
0.0354
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT TGAAACGATCGAAAAAGAGATGT 50.0 34.8 58.0 56.8 240-243 bp 1.0 51.5
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG TGAAACGATCGAAAAAGAGATGT 50.0 34.8 58.0 56.8 239-242 bp 1.0 51.5
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA TGAAACGATCGAAAAAGAGATGT 50.0 34.8 57.9 56.8 230-233 bp 1.0 50.8
trnL-UAG-ccsA_p4 GGTAGACACGCTGCTCTTAG TGAAACGATCGAAAAAGAGATGT 55.0 34.8 57.8 56.8 232-235 bp 1.0 50.2
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG TGAAACGATCGAAAAAGAGATGT 55.0 34.8 57.8 56.8 231-234 bp 1.0 50.2