Hotspot

ndhF-rpl32

SSC · 118830-119856 · 1027 bp

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Pi 0.0187
MarkerSeek score 63.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1027
variable_sites
52
pi
0.0187
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACGAAAGGTATGATCCACGC GTACGTTTCTTTGGAACCGC 50.0 50.0 58.1 57.7 1077-1095 bp 1.0 82.6
ndhF-rpl32_p2 CGAAAGGTATGATCCACGCA GTACGTTTCTTTGGAACCGC 50.0 50.0 58.1 57.7 1076-1094 bp 1.0 82.6
ndhF-rpl32_p3 CAAGTAGAAGTCCCACCCCT GTACGTTTCTTTGGAACCGC 55.0 50.0 58.3 57.7 1121-1139 bp 1.0 80.1
ndhF-rpl32_p4 ACGAAAGGTATGATCCACGC TACGTTTCTTTGGAACCGCC 50.0 50.0 58.1 58.8 1076-1094 bp 1.0 78.8
ndhF-rpl32_p5 CGAAAGGTATGATCCACGCA TACGTTTCTTTGGAACCGCC 50.0 50.0 58.1 58.8 1075-1093 bp 1.0 78.8