Hotspot

petA-psbJ

LSC · 68596-69618 · 1023 bp

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Pi 0.0121
MarkerSeek score 60.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1023
variable_sites
40
pi
0.0121
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1702-1726 bp 1.0 83.9
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1701-1725 bp 1.0 83.9
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC TTAGGAGGCCTCAATGACCA 50.0 50.0 58.0 58.0 1651-1675 bp 1.0 83.7
petA-psbJ_p4 CCGATGGGAGCAAGAGTAAC AACTGGCCGTTTTGATCCTT 55.0 45.0 58.1 58.0 1960-1984 bp 1.0 83.3
petA-psbJ_p5 CCGATGGGAGCAAGAGTAAC ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.1 58.0 1959-1983 bp 1.0 83.3