Hotspot

ycf3-trnS-GGA

LSC · 47916-48800 · 885 bp

Back to Sabia

Pi 0.00886
MarkerSeek score 59.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
885
variable_sites
21
pi
0.00886
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 966-977 bp 1.0 73.6
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 987-998 bp 1.0 73.0
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 937-948 bp 1.0 72.9
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA AACGTTGGCGAATTAGGTGA 47.6 45.0 58.0 57.8 1011-1022 bp 1.0 72.8
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA ACGTTGGCGAATTAGGTGAA 47.6 45.0 58.0 57.8 1010-1021 bp 1.0 72.8