Hotspot

psbC-trnS-UGA

LSC · 38649-38867 · 219 bp

Back to Sabia

Pi 0.0188
MarkerSeek score 59.80
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
219
variable_sites
8
pi
0.0188
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 323-326 bp 1.0 81.0
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GATGGCCGAGTGGTTGATAG 55.0 55.0 58.2 58.1 334-337 bp 1.0 80.3
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 269-272 bp 1.0 80.2
psbC-trnS-UGA_p4 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 366-369 bp 1.0 78.9
psbC-trnS-UGA_p5 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 365-368 bp 1.0 78.4