Hotspot

trnE-UUC-trnT-GGU

LSC · 33743-34539 · 797 bp

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Pi 0.0133
MarkerSeek score 63.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
797
variable_sites
27
pi
0.0133
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.91
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 834-907 bp 1.0 84.3
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 813-886 bp 1.0 84.2
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG GGGGTTTTGTGGAAAAAGCC 55.0 50.0 58.3 58.0 867-940 bp 1.0 84.0
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GGTTTTGTGGAAAAAGCCCC 55.0 50.0 58.3 58.0 865-938 bp 1.0 84.0
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA GGTTTTGTGGAAAAAGCCCC 50.0 50.0 57.7 58.0 844-917 bp 1.0 83.9