Hotspot

trnC-GCA-petN

LSC · 29613-30506 · 894 bp

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Pi 0.0114
MarkerSeek score 54.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
894
variable_sites
28
pi
0.0114
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTTGTATCGTTTTGGCGGC AAACGACCCCCTCTTTGAAC 45.0 50.0 57.9 58.0 1450-1462 bp 1.0 81.6
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AAACGACCCCCTCTTTGAAC 50.0 50.0 58.1 58.0 1394-1406 bp 1.0 81.5
trnC-GCA-petN_p3 TTTTGTATCGTTTTGGCGGC TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.9 58.0 1011-1013 bp 0.444444 59.0
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 955-957 bp 0.444444 58.8
trnC-GCA-petN_p5 AGGGGTTCAGTAATCCTCCC TTAAAGCAGCCCAAGCAAGA 55.0 45.0 58.1 58.0 1052-1054 bp 0.444444 58.5