Hotspot

trnK-UUU-rps16

LSC · 4360-5183 · 824 bp

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Pi 0.0104
MarkerSeek score 59.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
824
variable_sites
26
pi
0.0104
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGAGCTCAACCTACAGGAAC 50.0 55.0 58.2 57.6 936-957 bp 1.0 79.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGAGCTCAACCTACAGGAAC 50.0 55.0 58.2 57.6 935-956 bp 1.0 79.1
trnK-UUU-rps16_p3 TCCTAGCCGCACTTAAAAGC GGAGCTCAACCTACAGGAAC 50.0 55.0 58.3 57.6 951-972 bp 1.0 78.3
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGAGCTCAACCTACAGGAAC 50.0 55.0 58.3 57.6 945-966 bp 1.0 78.1
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGAGCTCAACCTACAGGAAC 50.0 55.0 57.5 57.6 922-943 bp 1.0 76.8