Hotspot

trnH-GUG-psbA

LSC · 79-457 · 379 bp

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Pi 0.0363
MarkerSeek score 74.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
379
variable_sites
34
pi
0.0363
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GTCGAAGCTCCATCCACAAA 55.0 50.0 58.1 58.2 343-439 bp 1.0 87.9
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GTCGAAGCTCCATCCACAAA 50.0 50.0 57.9 58.2 357-453 bp 1.0 87.7
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GTCGAAGCTCCATCCACAAA 50.0 50.0 57.9 58.2 356-452 bp 1.0 87.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GTCGAAGCTCCATCCACAAA 50.0 50.0 57.9 58.2 358-454 bp 1.0 87.6
trnH-GUG-psbA_p5 GCCTTGATCCACTTGGCTAC GTCGAAGCTCCATCCACAAA 55.0 50.0 58.6 58.2 348-444 bp 1.0 83.9