Hotspot

rpl16

LSC · 83811-85366 · 1556 bp

Pi 0.00209

MarkerSeek score 57.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1556
variable_sites: 13
pi: 0.00209
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	57.9	58.1	1577-1661 bp	1.0	44.6
rpl16_p2	GTCCAGAATAAACCTGCGGT	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.9	56.9	1576-1660 bp	1.0	44.6
rpl16_p3	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACC	50.0	32.0	57.9	56.6	1577-1661 bp	1.0	44.6
rpl16_p4	TCCGTCCAGAATAAACCTGC	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	57.6	58.1	1580-1664 bp	1.0	44.6
rpl16_p5	TCCGTCCAGAATAAACCTGC	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.6	56.9	1579-1663 bp	1.0	44.6