Hotspot

rbcL

LSC · 57670-59124 · 1455 bp

Pi 0.00142

MarkerSeek score 45.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1455
variable_sites: 8
pi: 0.00142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGAGTAGACCTTGTCGTTGT	TATTCGGTTCGGCTCAATCC	50.0	50.0	57.3	57.8	1595 bp	1.0	73.0
rbcL_p2	CGAGTAGACCTTGTCGTTGT	GGAAAAGATTGGGCCGAGTT	50.0	50.0	57.3	58.5	1565 bp	1.0	71.2
rbcL_p3	TCGAGTAGACCTTGTCGTTG	TATTCGGTTCGGCTCAATCC	50.0	50.0	57.0	57.8	1596 bp	1.0	70.7
rbcL_p4	TCAAAGGTTTCATTCACGCC	TATTCGGTTCGGCTCAATCC	45.0	50.0	56.9	57.8	1625 bp	1.0	69.9
rbcL_p5	CAAAGGTTTCATTCACGCCT	TATTCGGTTCGGCTCAATCC	45.0	50.0	56.9	57.8	1624 bp	1.0	69.9