Hotspot

psaC-ndhE

SSC · 120605-121273 · 669 bp

Back to Sabal

Pi 0.0036
MarkerSeek score 44.60
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
669
variable_sites
9
pi
0.0036
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGTTAGC 50.0 47.6 58.1 58.4 563-863 bp 1.0 73.0
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA GAAGCAGCTATTGGGTTAGC 50.0 50.0 58.1 56.6 562-862 bp 1.0 72.5
psaC-ndhE_p3 CAATACATCTGTGGGGCAGG TGAAGCAGCTATTGGGTTAGC 55.0 47.6 58.3 58.4 539-839 bp 1.0 71.5
psaC-ndhE_p4 CCAATACATCTGTGGGGCAG TGAAGCAGCTATTGGGTTAGC 55.0 47.6 58.3 58.4 540-840 bp 1.0 71.5
psaC-ndhE_p5 CAGGCTCGTACACATTGAGT TGAAGCAGCTATTGGGTTAGC 50.0 47.6 57.6 58.4 523-823 bp 1.0 71.1