Hotspot

psaJ-rpl33

LSC · 69576-70087 · 512 bp

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Pi 0.00182
MarkerSeek score 41.50
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
512
variable_sites
3
pi
0.00182
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TGGTTTGGGTCTTTAGCAGG TGACACCATTTCGGACACAA 50.0 45.0 57.7 57.7 658-692 bp 1.0 75.9
psaJ-rpl33_p2 TGGTTTGGGTCTTTAGCAGG TTGACACCATTTCGGACACA 50.0 45.0 57.7 57.7 659-693 bp 1.0 75.9
psaJ-rpl33_p3 GGTTTGGGTCTTTAGCAGGT TGACACCATTTCGGACACAA 50.0 45.0 57.7 57.7 657-691 bp 1.0 75.9
psaJ-rpl33_p4 GGTTTGGGTCTTTAGCAGGT TTGACACCATTTCGGACACA 50.0 45.0 57.7 57.7 658-692 bp 1.0 75.9
psaJ-rpl33_p5 TGGTTTGGGTCTTTAGCAGG ACACCATTTCGGACACAACT 50.0 45.0 57.7 57.7 656-690 bp 1.0 75.8