Hotspot

ndhC-trnV-UAC

LSC · 52059-53917 · 1859 bp

Back to Sabal

Pi 0.000326
MarkerSeek score 52.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1859
variable_sites
3
pi
0.000326
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAACCGGTGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1862-2003 bp 1.0 82.0
ndhC-trnV-UAC_p2 TAACCGGTGCTAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1854-1995 bp 1.0 81.9
ndhC-trnV-UAC_p3 TCCTTCACTAACCGGTGCTA AGCCCAATGCAACAAAATGC 50.0 45.0 58.1 58.5 1944-2085 bp 1.0 79.7
ndhC-trnV-UAC_p4 TAACCGGTGCTAAAACTCCG AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 1936-2077 bp 1.0 79.7
ndhC-trnV-UAC_p5 TCCTTCACTAACCGGTGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.1 57.5 1861-2002 bp 1.0 79.2