Hotspot

matK-trnK-UUU

LSC · 3268-4077 · 810 bp

Back to Sabal

Pi 0.00123
MarkerSeek score 49.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
810
variable_sites
5
pi
0.00123
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AGAAAGTGTTGTTGCCGAGA AACGGTAGAGTACTCGGCTT 45.0 50.0 57.7 58.2 887-890 bp 1.0 76.9
matK-trnK-UUU_p2 AGAAAGTGTTGTTGCCGAGA ACGGTAGAGTACTCGGCTTT 45.0 50.0 57.7 58.2 886-889 bp 1.0 76.9
matK-trnK-UUU_p3 AGAAAGTGTTGTTGCCGAGA GTCGTGCAGTGCAATTCAAT 45.0 45.0 57.7 57.7 977-980 bp 1.0 75.4
matK-trnK-UUU_p4 AAGTGTTGTTGCCGAGATCT AACGGTAGAGTACTCGGCTT 45.0 50.0 57.4 58.2 884-887 bp 1.0 75.1
matK-trnK-UUU_p5 AAGTGTTGTTGCCGAGATCT ACGGTAGAGTACTCGGCTTT 45.0 50.0 57.4 58.2 883-886 bp 1.0 75.1