Hotspot

rbcL

LSC · 53571-55001 · 1431 bp

Back to Rytidosperma

Pi 0.00839

MarkerSeek score 61.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 12
pi: 0.00839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGAGTAGACCTTGTCGTTGT	TCTCTTTCTAGTTCGCTTCT	50.0	40.0	57.3	53.5	1545 bp	1.0	40.6
rbcL_p2	CGAGTAGACCTTGTCGTTGT	TCTCTTTCTAGTTCGCTTCTT	50.0	38.1	57.3	54.4	1545 bp	1.0	40.6
rbcL_p3	CGAGTAGACCTTGTCGTTGT	TTCTCTTTCTAGTTCGCTTCT	50.0	38.1	57.3	54.4	1546 bp	1.0	40.6
rbcL_p4	TCGAGTAGACCTTGTCGTTG	TCTCTTTCTAGTTCGCTTCT	50.0	40.0	57.0	53.5	1546 bp	1.0	40.6
rbcL_p5	CGAGTAGACCTTGTCGTTGT	TCTCTTTCTAGTTCGCTTCTTT	50.0	36.4	57.3	55.2	1545 bp	1.0	40.6