Hotspot

psbB

LSC · 67359-68885 · 1527 bp

Back to Rytidosperma

Pi 0.00458

MarkerSeek score 61.30

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 7
pi: 0.00458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TCTGACACAGAATCCCCTAGA	AAAAGGACAGGAGGGGTTTC	47.6	50.0	57.2	57.3	1709 bp	1.0	60.8
psbB_p2	GACACAGAATCCCCTAGAAGG	AAAAGGACAGGAGGGGTTTC	52.4	50.0	57.2	57.3	1706 bp	1.0	60.3
psbB_p3	TCTGACACAGAATCCCCTAGAA	AAAAGGACAGGAGGGGTTTC	45.5	50.0	57.9	57.3	1709 bp	1.0	58.4
psbB_p4	TTCTGACACAGAATCCCCTAGA	AAAAGGACAGGAGGGGTTTC	45.5	50.0	57.9	57.3	1710 bp	1.0	58.4
psbB_p5	ACACAGAATCCCCTAGAAGG	AAAAGGACAGGAGGGGTTTC	50.0	50.0	55.9	57.3	1705 bp	1.0	58.3