Hotspot

petB

LSC · 69791-71223 · 1433 bp

Back to Rytidosperma

Pi 0.0056

MarkerSeek score 62.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1433
variable_sites: 8
pi: 0.0056
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1532-1543 bp	1.0	67.7
petB_p2	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1532-1543 bp	1.0	67.3
petB_p3	GGTAGTTCGACCGTGGATTT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1538-1549 bp	1.0	66.9
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1537-1548 bp	1.0	65.5
petB_p5	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATG	50.0	50.0	57.4	56.7	1537-1548 bp	1.0	65.1