Hotspot

rpl32-trnL-UAG

SSC · 102980-103302 · 323 bp

Back to Rytidosperma

Pi 0.0217

MarkerSeek score 51.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 323
variable_sites: 7
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAACGAGAATCCAAAACC	GAAGAATCACGCAATCCCCT	50.0	50.0	57.7	58.0	668 bp	1.0	77.8
rpl32-trnL-UAG_p2	GGCAACGAGAATCCAAAACC	GATCCGCACAAAAGAGTTGC	50.0	50.0	57.7	58.0	1007 bp	1.0	77.6
rpl32-trnL-UAG_p3	TCCCCTTTTTGAATGGCAGT	GAAGAATCACGCAATCCCCT	45.0	50.0	57.6	58.0	806 bp	1.0	77.3
rpl32-trnL-UAG_p4	GGCAACGAGAATCCAAAACC	ATGTCCCGAAGAAACCCAAC	50.0	50.0	57.7	58.1	741 bp	1.0	77.3
rpl32-trnL-UAG_p5	TCCCCTTTTTGAATGGCAGT	GATCCGCACAAAAGAGTTGC	45.0	50.0	57.6	58.0	1145 bp	1.0	77.1