Hotspot

ndhF-rpl32

SSC · 101771-102787 · 1017 bp

Back to Rytidosperma

Pi 0.00871
MarkerSeek score 65.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1017
variable_sites
8
pi
0.00871
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 1003-1100 bp 1.0 45.6
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 1004-1101 bp 1.0 45.6
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 1005-1102 bp 1.0 45.6
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 1006-1103 bp 1.0 45.6
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATCGAAGTACGT 55.0 35.0 58.1 53.2 1007-1104 bp 1.0 45.6