Hotspot

trnT-UGU-trnL-UAA

LSC · 45203-45908 · 706 bp

Back to Rytidosperma

Pi 0.0059

MarkerSeek score 66.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 4
pi: 0.0059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	797-806 bp	1.0	81.0
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	789-798 bp	1.0	80.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	767-776 bp	1.0	80.3
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.0	57.6	791-800 bp	1.0	79.7
trnT-UGU-trnL-UAA_p5	CGATGACCCTCGCATTACAA	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	783-792 bp	1.0	79.4