Hotspot

rpoC1-rpoC2

LSC · 24290-24482 · 193 bp

Back to Rytidosperma

Pi 0.0259

MarkerSeek score 67.40

Primer available yes

Label rpoC1-rpoC2

Feature Metadata

label_name: rpoC1-rpoC2
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 5
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoC2_p1	TCGAGGAAGCCATACAAGGA	TCGCAGTTCCGTCTATCTCT	50.0	50.0	58.1	58.0	298 bp	1.0	79.9
rpoC1-rpoC2_p2	TAGTCGGGCCTATTCGTACA	TCGCAGTTCCGTCTATCTCT	50.0	50.0	57.7	58.0	276 bp	1.0	78.4
rpoC1-rpoC2_p3	TCGGGCCTATTCGTACACTA	TCGCAGTTCCGTCTATCTCT	50.0	50.0	57.7	58.0	273 bp	1.0	78.4
rpoC1-rpoC2_p4	TCGAGGAAGCCATACAAGGA	TATGAAAGACCAGGTTGGCC	50.0	50.0	58.1	57.5	274 bp	1.0	76.3
rpoC1-rpoC2_p5	TCGAGGAAGCCATACAAGGA	GTTTCATCGCAGTTCCGTCT	50.0	50.0	58.1	58.6	304 bp	1.0	75.6