Hotspot

trnC-GCA-rpoB

LSC · 18221-18972 · 752 bp

Back to Rytidosperma

Pi 0.00798

MarkerSeek score 63.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 6
pi: 0.00798
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ACCCCTCGAATTGGATCTGA	50.0	50.0	58.1	58.1	862 bp	1.0	79.0
trnC-GCA-rpoB_p2	TAATCCATTGATCAGGCGGC	ACCCCTCGAATTGGATCTGA	50.0	50.0	58.1	58.1	903 bp	1.0	78.7
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCCCTCGAATTGGATCTGAC	50.0	55.0	58.1	57.8	861 bp	1.0	78.0
trnC-GCA-rpoB_p4	TAATCCATTGATCAGGCGGC	CCCCTCGAATTGGATCTGAC	50.0	55.0	58.1	57.8	902 bp	1.0	77.7
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	835 bp	1.0	77.4