Hotspot

trnG-GCC-trnfM-CAU

LSC · 12346-12669 · 324 bp

Back to Rytidosperma

Pi 0.0154

MarkerSeek score 61.90

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 5
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GTCGACCCAAGCGGATATAC	TCTACAGAGCGGAGTAGAGC	55.0	55.0	57.9	58.0	516-542 bp	1.0	79.9
trnG-GCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	TCTACAGAGCGGAGTAGAGC	50.0	55.0	58.1	58.0	476-502 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p3	CGTAGCGAGCGTAGTTCAAT	TCTACAGAGCGGAGTAGAGC	50.0	55.0	58.2	58.0	480-506 bp	1.0	79.1
trnG-GCC-trnfM-CAU_p4	TAGCGAGCGTAGTTCAATGG	TCTACAGAGCGGAGTAGAGC	50.0	55.0	57.8	58.0	478-504 bp	1.0	78.8
trnG-GCC-trnfM-CAU_p5	GTCGACCCAAGCGGATATAC	CGAGGCTCATAACCTTGAGG	55.0	55.0	57.9	57.8	482-508 bp	1.0	78.5