Hotspot

trnS-GCU-psbD

LSC · 7738-8752 · 1015 bp

Back to Rytidosperma

Pi 0.0101

MarkerSeek score 66.50

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 10
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1133-1160 bp	1.0	80.9
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1120-1147 bp	1.0	80.3
trnS-GCU-psbD_p3	ACGGAAAGAGAGGGATTCGA	GCCGGACCATCCTACAAAAA	50.0	50.0	58.2	58.2	1182-1209 bp	1.0	79.9
trnS-GCU-psbD_p4	GACGGAAAGAGAGGGATTCG	GCCGGACCATCCTACAAAAA	55.0	50.0	57.8	58.2	1183-1210 bp	1.0	79.4
trnS-GCU-psbD_p5	ACGGAAAGAGAGGGATTCGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.2	58.2	1169-1196 bp	1.0	79.3