Hotspot

rpl32-trnL-UAG

SSC · 116068-116948 · 881 bp

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Pi 0.0328
MarkerSeek score 72.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
881
variable_sites
28
pi
0.0328
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGAAATCCCTTTCTACGGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 940-963 bp 1.0 84.2
rpl32-trnL-UAG_p2 CGAAATCCCTTTCTACGGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 941-964 bp 1.0 84.2
rpl32-trnL-UAG_p3 CGAAATCCCTTTCTACGGGG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 950-973 bp 1.0 83.3
rpl32-trnL-UAG_p4 GCGTTGAAGGCTTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 962-985 bp 1.0 83.2
rpl32-trnL-UAG_p5 GCGTTGAAGGCTTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 963-986 bp 1.0 83.2