Hotspot

rps7-rps12

IRb · 99815-99867 · 53 bp

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Pi 0.0189
MarkerSeek score 63.30
Primer available yes
Label rps7-rps12

Feature Metadata

label_name
rps7-rps12
feature_type
igs
region
IRb
length_bp
53
variable_sites
1
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-rps12_p1 TGTAGTACCTCGACGTGACA CTGTGGAAAGCCGTATTCGA 50.0 50.0 57.8 58.0 179 bp 1.0 79.1
rps7-rps12_p2 TGTAGTACCTCGACGTGACA TCTGTGGAAAGCCGTATTCG 50.0 50.0 57.8 58.0 180 bp 1.0 79.1
rps7-rps12_p3 TGTAGTACCTCGACGTGACA GGCTTGGAGGGAGATCTTTC 50.0 55.0 57.8 57.7 143 bp 1.0 76.5
rps7-rps12_p4 TGTAGTACCTCGACGTGACA AGAATTCTGTGGAAAGCCGT 50.0 45.0 57.8 57.4 185 bp 1.0 74.5
rps7-rps12_p5 TCTGTAGTACCTCGACGTGA CTGTGGAAAGCCGTATTCGA 50.0 50.0 57.3 58.0 181 bp 1.0 74.4