Hotspot

rpl14-rpl16

LSC · 82867-82994 · 128 bp

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Pi 0.0397
MarkerSeek score 64.00
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
128
variable_sites
5
pi
0.0397
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 ATTCTCGAGCACCGCTATTG TTCTGGATTGATTATTTCTGGA 50.0 31.8 58.1 52.8 208 bp 0.5 20.0
rpl14-rpl16_p2 CAATTCTCGAGCACCGCTAT TTCTGGATTGATTATTTCTGGA 50.0 31.8 58.1 52.8 210 bp 0.5 20.0
rpl14-rpl16_p3 ATTCTCGAGCACCGCTATTG TTTCTGGATTGATTATTTCTGGA 50.0 30.4 58.1 53.7 209 bp 0.5 20.0
rpl14-rpl16_p4 CAATTCTCGAGCACCGCTAT TTTCTGGATTGATTATTTCTGGA 50.0 30.4 58.1 53.7 211 bp 0.5 20.0
rpl14-rpl16_p5 CACCGCTATTGTCTGCTACA TTCTGGATTGATTATTTCTGGA 50.0 31.8 57.7 52.8 199 bp 0.5 20.0